To more broadly assess the expression profiles of stem cell markers in CRC, we performed qRT-PCR analysis on 10 PDCO lines, analysing basal expression levels of CBC stem cell markers LGR5 and EPHB2, a quiescent stem cell marker BMI1, recently identified revival stem cell markers CLU and ANXA1, and a marker of differentiated intestinal cells, Keratin 20 (KRT20) (Figure 1D). Here, KRT20 is linked to colorectal carcinoma.