This effect was further investigated in vitro in human cancer cells where TGF-β-mediated EMT was reduced (as demonstrated by an increase in ZO, membrane β-catenin and E-cadherin) after silencing the NR: The culprit was found in the reduction of SNAIL1, whose expression is directly regulated by COUP-TFII [159]. This evidence concerns the gene TGFB1 and cancer.