HepG2 cells were pretreated with CT and/or compound C (AMPK Inhibitor) and Ex52735 (SIRT1 inhibitor) before treatment with ethanol and TG accumulation was evaluated to further examine the role of AMPK/SIRT1 in CT-mediated protection against ethanol-induced hepatic steatosis. The gene discussed is SIRT1; the disease is Hepatic steatosis.