IHC analysis revealed that beta-catenin accumulated in the nuclei of tumor cells mainly in the crypt area (Figure 7B) and quantitative evaluation of nucleus beta-catenin accumulation [29] further showed that nucleus beta-catenin H-score was significantly greater in mice receiving DMBA and tamoxifen compared to other groups (Figure 7C,D). This evidence concerns the gene CTNNB1 and neoplasm.