Differences and correlations were analysed for HIF-1α and ROS metabolic signalling pathway-related gene expression in normal tissues (GSM402687, GSM402688, GSM402689 and GSM402690) and osteosarcoma tissues (GSM402693, GSM402703, GSM402731 and GSM402747) from NCBI GEO datasets through R Package Limma and Affy. This evidence concerns the gene HIF1A and osteosarcoma.