Since the aberrant activation of PI3K-Akt signaling and RAF-MEK-ERK signaling pathway has been reported to be involved in colon cancer proliferation [19–21], we next detected the expression levels of some key proteins involved in PI3K-Akt and RAF-MEK-ERK signaling pathway by western blot assay in colon cancer cells upon co-treatment with Aspirin and Cisplatin. Here, AKT1 is linked to malignant colon neoplasm.