The mRNAs of brain tissue showed that the expression of GABRA1, GABRA6, St6galnac4, St8sia5, Pde9a, Tmem132a, Tmem163, Tmem62 and Tmem63c (Figure 5A and 5B) were up-regulated after over-expressed circNF1-419 in brain, which indicated that the central immune system were activated, in other words that circNF1-419 could activated central immune to improve the symptoms of AD, but need much more further study. This evidence concerns the gene PDE9A and Alzheimer disease.