In the training cohort, the univariate analysis suggested that age (P = .033), sex (P < .001), hepatitis (P < .001), AFP (P < .001), CA199 (P < .001), INR (P < .001), Neutrophil (P < .001), Platelet (P < .001), NLR (P = .018), PLR (P < .001), LMR (P = .023), and ANRI (P < .001) were potential differential factors between ICC and HCC (Table 2). The gene discussed is AFP; the disease is hepatitis A virus infection.