We provide also data that blocking the hyperactivation of PPP in macroH2A1 KD Huh-7 cells using PPP specific inhibitor Physcion led to restoration of downregulated cytokines (IL-6, IL-8) and nearly completely suppressed CD4+/CD25+/FoxP3+ Tregs, suggesting a previously unappreciated link between energy metabolism and chemokine/cytokine production in HCC cells. This evidence concerns the gene CD4 and hepatocellular carcinoma.