We found that a significantly higher number of cells immunopositive for β-galactosidase (β-gal), a key marker of cellular senescence, paralleled the progression of liver disease (control < cirrhosis < HCC) and the increased immunopositivity for macroH2A1 isoforms in a cohort of patients bearing differentiated/encapsulated tumors that we previously described 23, 24 (Figure S8). The gene discussed is MACROH2A1; the disease is Cirrhosis.