We next analyzed the expression of CARD3 in 24 non-metastatic CRC tissues and 32 metastatic CRC tissues from the same patients referenced in Figure 1C. The real-time PCR results showed that CARD3 expression in tumor tissues from metastatic CRC patients was higher than that in tumor tissues from patients with non-metastatic CRC (P < 0.001, Figure 4B). This evidence concerns the gene RIPK2 and colorectal carcinoma.