Mutations in the NLS and NES motifs of MSI1 were generated; Flag-control, Flag-tagged wild-type MSI1 (MSI1-wt), NES-mutant MSI1 (MSI1-NES-mut) and NLS-mutant MSI1 (MSI1-NLS-mut) were stably expressed in GBM cells (Figure 1E). Here, MSI1 is linked to glioblastoma.