An RNA immunoprecipitation (RIP) assay confirmed that VPS9D1-AS1 and miR-532-3p were substantially enriched in the immunoprecipitated AGO2 protein complex, suggesting that AGO2 bound to VPS9D1-AS1 and miR-532-3p directly in NSCLC cells (Figure 3H, P < 0.05). This evidence concerns the gene AGO2 and non-small cell lung carcinoma.