Moreover, astroglial lysosomal cholesterol accumulation (PFO/Lamp1 in GFAP+) significantly increased in hippocampus from AD and DS patients compared to controls (Figure 5B), an outcome that was more pronounced in DS (14.41±1.322 fold) than in AD brains (3.43±0.502 fold). Here, GFAP is linked to Alzheimer disease.