To investigate whether PL402 modulates ADEs level as we observed in vitro, we performed western blot analysis of hippocampal tissue from AD mice with or without PL402 treatment, the results showed that the expression of the two enzymes (MMP3 and MMP9) have significant up-regulation compared with the AD mice without PL402 treatment (Figure 5E, 5F). The gene discussed is MMP3; the disease is Alzheimer disease.