BCL2 and colorectal carcinoma: To further assess the relative changes of apoptosis and autophagy in CRC cells, western blotting was performed to investigate the effects of lycorine on the formation of autophagosomes and induction of apoptosis by evaluating the expression of LC3B-II and Beclin-1, two classical markers of autophagy, and Bax and Bcl-2, two sensitive markers of apoptosis.