While PTBP1 and TRA2B knockdown resulted in more DS-specific circRNAs, knockdown of MBNL1 only gave rise to 36 DS-specific compared to 60 DE-specific circRNAs (Fig. 5d), which indicates that the knockdown of these splicing factors may affect circRNA biogenesis through different mechanisms. Here, TRA2B is linked to Dravet syndrome.