Cells that meet the four indexes of CD133+, CD44+, CD24+, and EpCAM+ are defined as human liver cancer stem cells (hLCSCs), and cells that satisfy the four indexes of CD133-, CD44-, CD24-, and EpCAM are defined as non-hepatoma stem cells (non-hLCSCs). The gene discussed is PROM1; the disease is hepatocellular carcinoma.