By comparing peptide levels with publicly available tissue transcriptomics data sets, we identified increased expression of YWHAZ, ACTB, CAPZB, SPP1 and the collagen chains COL1A2, COL17A1, COL3A1 and COL5A2 in the CCA tumor both in the NCBI GEO and in the Whole Cancer Genome data sets and in agreement with our peptide profiles. The gene discussed is SPP1; the disease is cholangiocarcinoma.