The occurrence of 4 gene mutations in PCLO, GOLGA8J, EDRF1, and ASF1A on an initiator clone with TP53, PTEN, RLF, DLG2, and YY2 mutations might have resulted in the establishment of the founder clone, which then developed separately along germ cell and hematopoietic lines by adding GCT- and AML-specific gene mutations, respectively. This evidence concerns the gene ASF1A and acute myeloid leukemia.