To further support an indispensable role of CD44 in mediating SRGN-elicited function, we collected SRGN-containing conditioned medium from H1299/SRGN and H460/Sh-CTRL cells and incubated the conditioned medium with human duodenum adenocarcinoma HuTu80 cells in which the expression of CD44 was undetectable and HuTu80 cell clones stably expressing ectopically introduced CD44. The gene discussed is CD44; the disease is duodenal adenocarcinoma.