This finding was further supported by quantitative western blot analyses, which demonstrated increased levels of PTCH1 and GLI1 or CCND1 proteins upon tGLI2 and FHL2-GLI2 expression in all four cell models, although the increases in PTCH1 were not significant in the DAOY and BCC cells (Fig. 4d). Here, GLI1 is linked to skin basal cell carcinoma.