All three KRAS mutations were consistently identified in the CRC patients, although the VAFs of the three mutations were much lower than those found in the CRC tissue sample: CRC-1 (G12V), 1.613%; CRC-2 (G12C), 2.638%; and CRC-3 (G13D), 0.166% (Table 2, Fig. 2). This evidence concerns the gene KRAS and colorectal carcinoma.