To better understand the effect of upregulated S100A4 and ACKR3 expression on Doc-resistant PCa cell viability and migration, ACKR3 was knocked out using the crispr/cas9 method in DU145R and PC3R cells and/or S100A4 expression of knocked down through transfection of targeted shRNA. This evidence concerns the gene ACKR3 and posterior cortical atrophy.