TSC2 and neoplasm: This AMPK inhibitory effect on mTOR in tumor cells occurs by two distinct pathways: (1) AMPK phosphorylates TSC2 at Ser1345, which stimulates its Rheb-GAP activity; (2) AMPK phosphorylates Raptor at Ser722 and Ser792 which are both reasonably well conserved throughout eukaryotes [52–54].