The results showed that there are 12 mRNAs were associated with prognosis of COAD patients, where KRTAP3-1, SLC35F3 and SLITRK4 were upregulated in both N stage and lymphatic invasion, while ATP2B2, CILP, ELOVL2, ERBB4, PCDH9, RBM20, SPTBN4, SYT6 and TMEM132E were upregulated in N stage. This evidence concerns the gene ERBB4 and colon adenocarcinoma.