We also found the increase of p62 by shikonin in both of the two cell lines were much higher when interfered the expression of galectin-1 and the shikonin induced cleavage of LC3 was decrease, which means the downregulation of galectin-1 and the dimer may hinder the autophage progress of CRC cell lines and promote the accumulation of p62. The gene discussed is SQSTM1; the disease is colorectal carcinoma.