Other parameters such as expression of CD40 on APCs (r = 0.829; p = 0.17), ICOSL on APCs (r = 0.732; p = 0.27) (Fig. 6A), the proportion of PD-1high CD8+ T cells (r = − 0.308; p = 0.61) (Fig. 6B), the proportion of tumor cells (r = 0.656; p = 0.23), and the proportion of B7-H4-expressing tumor cells (r = 0.665; p = 0.22) (Fig. 6C) were not significantly correlated between tumor sites. Here, CD40 is linked to neoplasm.