Our results support our hypothesis that the β-cryptoxanthin-induced cytotoxicity against cervical cancer HeLa cells is linked to (1) enhanced ROS generation, (2) upregulation of caspase-3, -7, and -9, Bax, and p-53 at the mRNA level, with concordant downregulation of Bcl-2, (3) nuclear condensation and significant loss of the integrity of the mitochondrial membrane, (4) enhanced activation of caspase-3, and finally, (5) cleavage of nuclei DNA. Here, TP53 is linked to cervical cancer.