Moreover, the results also indicated that the expression levels of P-LKB1, P-AMPKa12, P-AMPKa2, GLUT4 mRNA, and proteins reduced significantly in the model group, showing that the LKB1–AMPK–GLUT4 signaling cascade was not only inhibited markedly under the pathological condition of diabetes, but also contributed to the development of diabetes. This evidence concerns the gene PRKAA2 and diabetes mellitus.