HAND2 and neoplasm: RT-qPCR done to verify the overexpression of HAND2-AS1 in mice tumors (Fig. 8a) confirmed that expression of HAND2-AS1 was indeed increased in the tumor injected with cells expressing oe-HAND2-AS1 + NC mimic in comparison to oe-NC + NC mimic treatment (p < 0.05), suggesting successful transfection.