AGI-134 did not protect mice from distal tumor development that were non-immunized, and thus anti-Gal negative, but it did protect those that were expressing anti-Gal (Fig. 4c), demonstrating that the abscopal effect induced by AGI-134 in the B16-F10 model was dependent on the interaction of anti-Gal with the α-Gal portion of AGI-134. Here, GAL is linked to neoplasm.