Our results revealed that the expression levels of TGF-β, phosphorylated-Smad2 (p-Smad2) and phosphorylated-Smad3 (p-Smad3) were decreased with ENO1 knockdown, whereas the total Smad2 and Smad3 expression levels were not significantly different between the normal glucose and hyperglycemia groups (Fig. 5a, b). The gene discussed is ENO1; the disease is Hyperglycemia.