Our results revealed that the expression levels of TGF-β, phosphorylated-Smad2 (p-Smad2) and phosphorylated-Smad3 (p-Smad3) were decreased with ENO1 knockdown, whereas the total Smad2 and Smad3 expression levels were not significantly different between the normal glucose and hyperglycemia groups (Fig. 5a, b). Here, SMAD3 is linked to Hyperglycemia.