As shown in Fig. 4b-e, compared with the results in control group, the expression of Lyn (P = 0.013), p-Akt (P = 0.009), NF-κB nuclei (P = 0.037) and tumor necrosis factor-α (TNF-α, P = 0.040) in the lung tissues of the cold stress group rats was significantly elevated, and the expression of them in the rats that received 30 mg/kg AF was significantly reduced. This evidence concerns the gene TNF and atrial fibrillation.