Assessing NETosis by means of the cell impermeable dye Sytox Green [41] did however not reveal differences between WT or Il23a-/- neutrophils that were isolated from infected mice at 24h post infection and put in contact with C. albicans hyphae in vitro(S7B Fig), suggesting that NETosis does not contribute to the IL-23-dependent cell death phenotype. The gene discussed is IL23A; the disease is infection.