Ethanol-induced gastric model was used to explore the beneficial effect of Manilkara zapota extract through a variety of mechanisms that stimulate ulcers including free radicals OH−, NO production, mucus exhaustion, mucosal damage, release of superoxide anion, which ultimately prolonged the tissue oxidative stress, and release of inflammatory mediators like TNF-α, p-NFκB, and COX-2, which are measured through the proteomic analysis. This evidence concerns the gene TNF and ulcer disease.