In addition, complementing RNA profiling using the nCounter nanoString technology was performed on LCM dissected FFPE liver explant samples from 10 PSC patients with cholestatic liver disease (disease group), 8 patients without PSC (control group), as well as 2 siblings from the pediatric/neonatal cholestatic liver disease group with a homozygous mutation in the ABCB4 gene (p.H1238Y). The gene discussed is ABCB4; the disease is pancreatic serous cystadenoma.