To understand whether β-catenin is related to YAP nuclear localization in melanoma-associated fibroblasts, we collected tumor tissues from mice bearing BrafV600E; Ptenlox5-Fb or BrafV600E; Ptenlox5-bcat/Fb melanoma14 for double staining using an anti-YAP antibody and an anti-α-SMA antibody. Here, ACTA1 is linked to neoplasm.