As shown in Fig. 3l, there were 2.81 ± 0.55% PI-positive; Annexin V-positive melanoma cells and 5.44 ± 0.32% PI-positive; Annexin V-negative melanoma cells in SK-MEL-24; GFP/Fb spheroids; however, there were 3.47 ± 1.17% PI-positive; Annexin V-positive melanoma cells and 7.00 ± 1.78% PI-positive; Annexin V-negative melanoma cells in SK-MEL-24; bcat-GFP/Fb spheroids. This evidence concerns the gene ANXA5 and melanoma.