PBX1 and hydrops fetalis: Oil Red O and Alizarin Red S staining showed that ectopic NANOG expression or PBX1 overexpression decreased lipid droplet accumulation by 0.71 ± 0.14- and 0.62 ± 0.13-fold, respectively, in comparison to that of the vector group (P < 0.05 and P < 0.01, respectively) and also increased calcium nodal formation in HF-MSCs by 2.18 ± 0.05- and 1.78 ± 0.18-fold (P < 0.001 and P < 0.01), respectively (Figures 3(a)–3(d)).