Due to the fact that Hsp90 isoforms had different expression in obese and overweight NAFLD patients (beta isoform was upregulated while alpha isoform was downregulated), it was also decided to calculate the ratio between Hsp90α and Hsp90β and analyze its diagnostic accuracy for NAFLD compared to the individual biomarkers. Here, HSP90AA1 is linked to metabolic dysfunction-associated steatotic liver disease.