So, we treated miR-28-5p sponge HCC cells and its control cells with special IGF-1 siRNA and found that the difference in the proportion of liver CSCs, self-renewal ability, and tumorigenesis capacity between miR-28-5p knockdown and control hepatoma cells was diminished by special IGF-1 siRNA (Figures 4(k)–4(m)). The gene discussed is IGF1; the disease is hepatocellular carcinoma.