As flow cytometry data indicated a CMV-driven proliferation of CD8+γδ T cells that displayed effector phenotype and preferentially enriched with Vγ9−γδ T cells, we, therefore, sought to characterize the TRG CDR3 clonotypes of γδ T cells to determine if there are differences regarding TCR diversity or clonal focusing driven by CMV infection. This evidence concerns the gene CD8A and cytomegalovirus infection.