To better understand the mechanism of Bag-1-mediated cell survival in breast cancer cell lines, we investigated phosphorylation levels of Bad protein in MCF-7, BT-474 and MDA-MB-231 cells after transfection with Bag-1 plasmid or Bag-1 siRNA (Fig. 4a, Additional file 1: Figure S6). The gene discussed is BAD; the disease is breast cancer.