FH and pneumococcal meningitis: Brain FH levels were determined by ELISA and were significantly higher in mice with pneumococcal meningitis at 6 (median 6.92 μg/mg tissue, P = 0.008), 24 (median 13.89 μg/mg tissue, P = 0.008) and 48 h (median 3.13 μg/mg tissue, P = 0.016) after infection compared to saline-inoculated mice (median 0.93 μg/mg tissue, Fig. 3a).