However, when kept ≥26 weeks on a diet high in fat, fructose, and cholesterol (the Amylin liver NASH diet; AMLN [20, 21]), C57BL/6J mice have been shown to develop the hepatic pathological hallmarks of NASH, including steatosis, lobular inflammation, and ballooning degeneration, as well as mild to moderate fibrosis [21–26]. Here, IAPP is linked to metabolic dysfunction-associated steatohepatitis.