We could detect lower levels in all IgG subclasses as well as in IgA and IgM, although the most dramatic differences were seen in IgG and particularly in IgG1 sequences (Fig. 3, Table 2, Supplemental Fig. 11), with an average number of 15.2 SHM compared to the closest germline in RA and 19.9 SHM in sequences from healthy individuals (p < 0.0001). This evidence concerns the gene CD40LG and rheumatoid arthritis.