The expression pattern of Gr-1+ (Ly6C/Ly6G), CD44+, and IL-17A+ cells within the cloud of CD11b+ myeloid cells were uniquely high in the spleen of 4T1 breast cancer bearing mice (Figure 5) in accordance with the splenomegaly shown in Figure 2D. On the contrary, the B220 and CD62L markers within the cloud of CD19+ B-cells, and the CD62L within the cloud of CD4+ and CD8+ T-cells also were highly reduced in the spleen of 4T1 tumorous mice (Figure 5). This evidence concerns the gene CD8A and breast cancer.