The results of ROC curve indicated that NPM1, TRIP6, HSP90B1, UBC, FN1, RPL14, UBB and EEF1A1 could distinguish male CAD samples from female CAD tissues best, among all the up and down regulated hub genes (NPM1: AUC = 1; ATM: AUC = 0.875; TRIP6: AUC = 1; HSP90B1: AUC = 1, HIST1H1C: AUC = 0.938; UBC: AUC = 1; FN1: AUC = 1; RPL14: AUC = 1; UBB: AUC = 1; EEF1A1: AUC = 1) (Figure 18). The gene discussed is FN1; the disease is coronary artery disorder.