MGMT and neoplasm: Additionally, the methylation status of O6-methylguanine-DNA methyltransferase (MGMT) has been implicated as a useful biomarker for conferring tumor resistance to alkylating chemotherapies; methylation of the MGMT promoter leads to transcriptional silencing of MGMT, which is associated with loss of MGMT expression and increased response to alkylating chemotherapies such as temozolomide (TMZ) [9].