The results showed that CRISPRi mediated stable depletion of DIRC3 in A375 and 501mel melanoma cells, using either DIRC3sg1 or DIRC3sg2 to target dCas9-KRAB to the DIRC3 promoter, also resulted in significantly increased colony formation in soft agar compared to control (Fig 5C and 5D). This evidence concerns the gene DIRC3 and melanoma.