Reduction of MITF led to increased DIRC3 expression in both 501mel and SK-MEL-28, but not in A375 cells (Fig 2B), indicating that DIRC3 is transcriptionally repressed by MITF in at least a subset of melanoma cells, whilst SOX10 knockdown using two independent siRNAs increased DIRC3 expression in all three melanoma cell lines tested (Fig 2C). The gene discussed is SOX10; the disease is melanoma.