TGFB1 and renal fibrosis: To explore whether down-regulating of ER-resident SELENOS by HG and TGF-β1 on HMCs effect on renal fibrosis, we knocked down SELENOS by SiRNA and found reduced SELENOS mRNA could decrease fibronectin expression on HMCs, which indicates that down-regulating of ER-resident SELENOS plays protective effect on renal fibrosis.